The Trans-Blot SD Semi-Dry Electrophoretic Blot Cell includes the cell, a Trans-Blot SD agarose gel support frame, and four packs of extra-thick blotting paper (60 sheets each, 7 x 8.4 cm, 8 x 13.5 cm and 30 sheets each, 14 x 16 cm, and 18 x 18.5 cm). The safety cover cuts off electric current when lifted, preventing electric shock.
Trans-Blot Turbo system delivers efficient and reproducible transfer of proteins up to 400 kD in as little as 3 minutes. Traditional semi-dry transfer system transfers proteins ranging from 10100 kD in 1560 minutes. The additional plastic templates required by other semi-dry blotters to prevent electrode shorts are unnecessary. Trans-Blot SD Semi-Dry Blotting Apparatus.
Platinum coated titanium anodic plate electrodes and stainless steel cathode plate electrodes provide smooth and reliable transfers, durability and long life. The one-step locking system allows for quick and easy installation. Tank blotching or semi-dry blotting Tank blotting advocated needed high-capacity, small pore-size membrane pH of buffer may been critical Native, non-denaturing Depends on pH of gel output and pear a protein of interest Tank blotting recommended temper regulation may be needed to maintain activity 0. Minimal buffer requirements save money Ability to transfer up to four precast or hand-poured Mini-PROTEAN® gels, or three Criterion™ gels. Features and Benefits Transfers are completed in just 15-60 minutes. SD semi-dry transfer cell allows fast, efficient. Then click on the Literature (downloads only) to find the manual.The Trans-Blot Semi-Dry (SD) Transfer Cell provides fast, efficient, and cost-effective transfer without buffer reservoirs or gel cassettes. BIO-RAD TRANS-BLOT SD SEMI DRY TRANSFER CELL FOR WESTERN BLOTTING, NUCLEIC ACID, DNA & RNA TRANSFER. On the Trans-Blot SD Semi-Dry Transfer Cell page click on the document tab below the picture of the system. Of protein may be transferred through the membrane and onto the filter paper below. Under the strong fieldsĭeveloped by this apparatus, transfers may not always be quantitative. The Trans-Blot Turbo system uses proprietary Turbo technology to transfer proteins up to 25 times faster than traditional Western blotting methods. Recommended to prevent excessive heating during the run. A current limit (3 mA/cm2 for large gels 5.5 mA/cm2 for mini gels) is Large gelsĬan be transferred for 30 minutes to 1 hour at 15–25 V. The minimal amount of buffer required for the transfer (about 250 ml per experiment), saves money and reagents. Transfer mini gels for 15–30 minutes at 10–15 V. The Bio-Rad Trans-Blot Semi-Dry System provides an efficient way to transfer proteins to nitrocellulose membranes with less preparation time, material use or clean-up as compared to wet transfer cell counterparts.
Bio rad western blot trans blot semi dry manual#
Although the Bio-Rad and Novex instructions don't seem to be that different (once again, see below) I think your best bet would be to follow the Novex instructions since they have probably optimized conditions for their apparatus.įrom the Trans-Blot SD Semi-Dry Transfer Cell, Rev C Instruction Manual (Part 1703940)
For most proteins, I usually transfer at 15 V for 25 - 30 min as recommended by Bio-Rad (see below).
Bio rad western blot trans blot semi dry full#
I occasionally use the Bio-Rad Trans-blot SD for my transfers. Trans-Blot® Semi-Dry Quick Reference Guide Catalog Number 170-3940 Full instructions are provided in the manual and should be read before using the Trans.
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